Soil pH is one of the most critical properties that influence soil health and plant growth. It plays a key role in determining the availability of essential nutrients to crops, making it an important factor in assessing soil fertility and guiding fertilizer application. Alongside other soil tests, measuring pH is an essential step in understanding the overall condition of the soil.
There are two primary methods for measuring soil pH: potentiometric and colorimetric. With advancements in analytical technology, most modern laboratories now use the potentiometric method due to its accuracy, speed, and ease of use. This method is widely used in the ASI (Agricultural Soil Investigation) procedure. The basic principle involves a pH meter, which measures the potential difference generated by the activity of hydrogen ions (Hâº) inside and outside a glass electrode. The relationship can be expressed as:
Where:
- a₠represents the H⺠ion activity inside the glass electrode, which remains constant.
- a₂ refers to the H⺠ion activity in the solution being tested.
From this equation, it's clear that the potential difference depends solely on the H⺠activity in the test solution. The negative logarithm of this value gives the pH, which can be directly read from the pH meter after it has been properly calibrated.
To ensure accurate measurements, standard buffer solutions are used during calibration. These include:
1. **pH 4.01 Standard Buffer Solution**: Dissolve 10.21 g of potassium hydrogen phthalate (KHC₈H₄O₄, analytical grade) in 1000 ml of distilled water after drying at 105°C.
2. **pH 6.87 Standard Buffer Solution**: Dissolve 3.39 g of potassium dihydrogen phosphate (KH₂PO₄, analytical grade) in 1000 ml of distilled water after drying at 50°C.
3. **pH 9.18 Standard Buffer Solution**: Dissolve 3.39 g of borax (Na₂B₄O₇·10H₂O, analytical grade) in 1000 ml of distilled water.
**Calibration Steps**
Before testing, the pH meter must be calibrated using two different standard buffers. For example, you might use pH 4.01 and 6.87 or 6.87 and 9.18, depending on the expected soil pH. Insert the electrode into the first buffer, turn on the meter, adjust the temperature compensation, and set the reading to match the known pH of the buffer. This process corrects for any electrode asymmetry. After calibrating with the first buffer, check the second one. If the deviation exceeds 0.02 units, the electrode may need replacement or further inspection.
**Measuring Soil pH**
To measure the pH of a soil sample, take 10 ml of the sample and add 25 ml of boiled, cooled distilled water using an extract dispenser. Mix the mixture thoroughly on a blender for 10 minutes, then let it settle for 30 minutes. Finally, insert the electrode into the suspension and record the pH value directly from the meter. This reading provides an accurate representation of the soil’s acidity or alkalinity, helping in making informed agricultural decisions.
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